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ratp  (New England Biolabs)


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    Structured Review

    New England Biolabs ratp
    Ratp, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 99/100, based on 33702 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/ratp/T4+DNA+Ligase/10__55730_slash_1300___0179__3240-55-27-32
    Average 99 stars, based on 33702 article reviews
    ratp - by Bioz Stars, 2026-10
    99/100 stars

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    Related Articles

    Purification:

    Article Title: Targeting of microRNA-22 Suppresses Tumor Spread in a Mouse Model of Triple-Negative Breast Cancer.
    Article Snippet: The lysate was processed using the PureLink RNA Mini Kit (Thermo Fisher) using the instructions provided in the kit. .. The purified RNA was subjected to Poly (A) tailing by using E. coli Poly (A) Polymerase and rATP from New England BioLabs following the manufacturer’s instructions. .. Ten μL of the poly (A) tailed-RNA was reverse-transcribed using the SuperScriptTM IV First-Strand Synthesis System (Thermo Fisher) following the manufacturer’s instructions.

    Article Title: Targeting of microRNA-22 Suppresses Tumor Spread in a Mouse Model of Triple-Negative Breast Cancer
    Article Snippet: The lysate was processed using the PureLink RNA Mini Kit (Thermo Fisher) using the instructions provided in the kit. .. The purified RNA was subjected to Poly (A) tailing by using E. coli Poly (A) Polymerase and rATP from New England BioLabs following the manufacturer’s instructions. .. Ten µL of the poly (A) tailed-RNA was reverse-transcribed using the SuperScript™ IV First-Strand Synthesis System (Thermo Fisher) following the manufacturer’s instructions.

    Sequencing:

    Article Title: SAG-RAD: A Method for Single-Cell Population Genomics of Unicellular Eukaryotes
    Article Snippet: The digestion reaction was incubated at 37 °C for 60 min, then inactivated at 80 °C for 20 min and slowly cooled to room temperature for 45 min using a thermocycler (Veriti 96 well; Applied Biosystems). .. For P1 sequencing adapter ligation, 1 μl of 10× NEB2 buffer (New England Biolabs), 5 μl ultra-pure water, 3 μl of 100 nM barcoded P1 adapter, 0.6 μl of 100 nM rATP, and 0.5 μl of 200 U/μl T4 DNA ligase (New England Biolabs) were added to each RAD library tube. .. The ligation reaction was incubated at 20 °C for 60 min, then inactivated at 65 °C for 30 min and slowly cooled to room temperature for 45 min using a thermocycler (Veriti 96 well; Applied Biosystems).

    Adapter Ligation:

    Article Title: SAG-RAD: A Method for Single-Cell Population Genomics of Unicellular Eukaryotes
    Article Snippet: The digestion reaction was incubated at 37 °C for 60 min, then inactivated at 80 °C for 20 min and slowly cooled to room temperature for 45 min using a thermocycler (Veriti 96 well; Applied Biosystems). .. For P1 sequencing adapter ligation, 1 μl of 10× NEB2 buffer (New England Biolabs), 5 μl ultra-pure water, 3 μl of 100 nM barcoded P1 adapter, 0.6 μl of 100 nM rATP, and 0.5 μl of 200 U/μl T4 DNA ligase (New England Biolabs) were added to each RAD library tube. .. The ligation reaction was incubated at 20 °C for 60 min, then inactivated at 65 °C for 30 min and slowly cooled to room temperature for 45 min using a thermocycler (Veriti 96 well; Applied Biosystems).



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